Alternative Quantiferon cytokines for diagnosis of children with active tuberculosis and HIV co-infection in Ghana.
Identifieur interne : 000103 ( Main/Exploration ); précédent : 000102; suivant : 000104Alternative Quantiferon cytokines for diagnosis of children with active tuberculosis and HIV co-infection in Ghana.
Auteurs : Christian Lundtoft [Allemagne] ; Anthony Afum-Adjei Awuah [Ghana] ; Norman Nausch [Allemagne] ; Anthony Enimil [Ghana] ; Ertan Mayatepek [Allemagne] ; Ellis Owusu-Dabo [Ghana] ; Marc Jacobsen [Allemagne]Source :
- Medical microbiology and immunology [ 1432-1831 ] ; 2017.
Abstract
IFN-γ release assays (IGRAs) often present false-negative or indeterminate results in children with tuberculosis. HIV co-infection may contribute to decreased sensitivity of IGRAs by impairing T-cell IFN-γ expression. Measurement of alternative cytokines in QuantiFERON(®) (QFT) supernatants can circumvent the IFN-γ-dependency and may improve QFT sensitivity. We aimed to identify additional cytokines from QFT supernatants for detection of Mycobacterium tuberculosis infection in children with tuberculosis and HIV co-infection from Ghana. Concentrations of 18 cytokines in QFT supernatants from children (0-16 years) with tuberculosis concomitantly infected with HIV (n = 25) or without HIV (n = 24) from Ghana were measured using cytometric bead array (CBA). 29% of the children showed positive IFN-γ test results, and five cytokines, i.e., IL-6, IL-21, TNF-α, IL-1α and IP-10, detected M. tuberculosis infection with comparable or, for IL-6, with significantly higher sensitivity (59%). Increased age and HIV co-infection were associated with decreased cytokine induction, and especially IL-21 and IP-10 were less prevalent in HIV co-infected children with tuberculosis. Combined cytokine analyses increased proportions of positive tests, and a four-cytokine subset (i.e., IL-6, IL-21, IFN-γ, IL-1α) predicted 78% of the children with tuberculosis correctly. Combined evaluation of IFN-γ and alternative cytokines improved IGRA-sensitivity in children with tuberculosis.
DOI: 10.1007/s00430-017-0501-6
PubMed: 28299430
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000172
- to stream PubMed, to step Curation: 000172
- to stream PubMed, to step Checkpoint: 000172
- to stream Ncbi, to step Merge: 000B05
- to stream Ncbi, to step Curation: 000B05
- to stream Ncbi, to step Checkpoint: 000B05
- to stream Main, to step Merge: 000103
- to stream Main, to step Curation: 000103
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Alternative Quantiferon cytokines for diagnosis of children with active tuberculosis and HIV co-infection in Ghana.</title><author><name sortKey="Lundtoft, Christian" sort="Lundtoft, Christian" uniqKey="Lundtoft C" first="Christian" last="Lundtoft">Christian Lundtoft</name><affiliation wicri:level="3"><nlm:affiliation>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Awuah, Anthony Afum Adjei" sort="Awuah, Anthony Afum Adjei" uniqKey="Awuah A" first="Anthony Afum-Adjei" last="Awuah">Anthony Afum-Adjei Awuah</name><affiliation wicri:level="1"><nlm:affiliation>Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi, Ghana.</nlm:affiliation><country xml:lang="fr">Ghana</country><wicri:regionArea>Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi</wicri:regionArea><wicri:noRegion>Kumasi</wicri:noRegion></affiliation></author><author><name sortKey="Nausch, Norman" sort="Nausch, Norman" uniqKey="Nausch N" first="Norman" last="Nausch">Norman Nausch</name><affiliation wicri:level="3"><nlm:affiliation>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Enimil, Anthony" sort="Enimil, Anthony" uniqKey="Enimil A" first="Anthony" last="Enimil">Anthony Enimil</name><affiliation wicri:level="1"><nlm:affiliation>Komfo Anokye Teaching Hospital (KATH), Kumasi, Ghana.</nlm:affiliation><country xml:lang="fr">Ghana</country><wicri:regionArea>Komfo Anokye Teaching Hospital (KATH), Kumasi</wicri:regionArea><wicri:noRegion>Kumasi</wicri:noRegion></affiliation></author><author><name sortKey="Mayatepek, Ertan" sort="Mayatepek, Ertan" uniqKey="Mayatepek E" first="Ertan" last="Mayatepek">Ertan Mayatepek</name><affiliation wicri:level="3"><nlm:affiliation>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Owusu Dabo, Ellis" sort="Owusu Dabo, Ellis" uniqKey="Owusu Dabo E" first="Ellis" last="Owusu-Dabo">Ellis Owusu-Dabo</name><affiliation wicri:level="1"><nlm:affiliation>Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi, Ghana.</nlm:affiliation><country xml:lang="fr">Ghana</country><wicri:regionArea>Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi</wicri:regionArea><wicri:noRegion>Kumasi</wicri:noRegion></affiliation></author><author><name sortKey="Jacobsen, Marc" sort="Jacobsen, Marc" uniqKey="Jacobsen M" first="Marc" last="Jacobsen">Marc Jacobsen</name><affiliation wicri:level="3"><nlm:affiliation>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf, Germany. marc.jacobsen@med.uni-duesseldorf.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28299430</idno><idno type="pmid">28299430</idno><idno type="doi">10.1007/s00430-017-0501-6</idno><idno type="wicri:Area/PubMed/Corpus">000172</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000172</idno><idno type="wicri:Area/PubMed/Curation">000172</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000172</idno><idno type="wicri:Area/PubMed/Checkpoint">000172</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000172</idno><idno type="wicri:Area/Ncbi/Merge">000B05</idno><idno type="wicri:Area/Ncbi/Curation">000B05</idno><idno type="wicri:Area/Ncbi/Checkpoint">000B05</idno><idno type="wicri:Area/Main/Merge">000103</idno><idno type="wicri:Area/Main/Curation">000103</idno><idno type="wicri:Area/Main/Exploration">000103</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Alternative Quantiferon cytokines for diagnosis of children with active tuberculosis and HIV co-infection in Ghana.</title><author><name sortKey="Lundtoft, Christian" sort="Lundtoft, Christian" uniqKey="Lundtoft C" first="Christian" last="Lundtoft">Christian Lundtoft</name><affiliation wicri:level="3"><nlm:affiliation>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Awuah, Anthony Afum Adjei" sort="Awuah, Anthony Afum Adjei" uniqKey="Awuah A" first="Anthony Afum-Adjei" last="Awuah">Anthony Afum-Adjei Awuah</name><affiliation wicri:level="1"><nlm:affiliation>Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi, Ghana.</nlm:affiliation><country xml:lang="fr">Ghana</country><wicri:regionArea>Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi</wicri:regionArea><wicri:noRegion>Kumasi</wicri:noRegion></affiliation></author><author><name sortKey="Nausch, Norman" sort="Nausch, Norman" uniqKey="Nausch N" first="Norman" last="Nausch">Norman Nausch</name><affiliation wicri:level="3"><nlm:affiliation>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Enimil, Anthony" sort="Enimil, Anthony" uniqKey="Enimil A" first="Anthony" last="Enimil">Anthony Enimil</name><affiliation wicri:level="1"><nlm:affiliation>Komfo Anokye Teaching Hospital (KATH), Kumasi, Ghana.</nlm:affiliation><country xml:lang="fr">Ghana</country><wicri:regionArea>Komfo Anokye Teaching Hospital (KATH), Kumasi</wicri:regionArea><wicri:noRegion>Kumasi</wicri:noRegion></affiliation></author><author><name sortKey="Mayatepek, Ertan" sort="Mayatepek, Ertan" uniqKey="Mayatepek E" first="Ertan" last="Mayatepek">Ertan Mayatepek</name><affiliation wicri:level="3"><nlm:affiliation>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author><author><name sortKey="Owusu Dabo, Ellis" sort="Owusu Dabo, Ellis" uniqKey="Owusu Dabo E" first="Ellis" last="Owusu-Dabo">Ellis Owusu-Dabo</name><affiliation wicri:level="1"><nlm:affiliation>Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi, Ghana.</nlm:affiliation><country xml:lang="fr">Ghana</country><wicri:regionArea>Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR), Kumasi</wicri:regionArea><wicri:noRegion>Kumasi</wicri:noRegion></affiliation></author><author><name sortKey="Jacobsen, Marc" sort="Jacobsen, Marc" uniqKey="Jacobsen M" first="Marc" last="Jacobsen">Marc Jacobsen</name><affiliation wicri:level="3"><nlm:affiliation>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf, Germany. marc.jacobsen@med.uni-duesseldorf.de.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Pediatric Infectious Diseases Group, Department of General Pediatrics, Neonatology, and Pediatric Cardiology, University Children's Hospital, Moorenstr. 5, 40225, Duesseldorf</wicri:regionArea><placeName><region type="land" nuts="1">Rhénanie-du-Nord-Westphalie</region><region type="district" nuts="2">District de Düsseldorf</region><settlement type="city">Düsseldorf</settlement></placeName></affiliation></author></analytic><series><title level="j">Medical microbiology and immunology</title><idno type="eISSN">1432-1831</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">IFN-γ release assays (IGRAs) often present false-negative or indeterminate results in children with tuberculosis. HIV co-infection may contribute to decreased sensitivity of IGRAs by impairing T-cell IFN-γ expression. Measurement of alternative cytokines in QuantiFERON(®) (QFT) supernatants can circumvent the IFN-γ-dependency and may improve QFT sensitivity. We aimed to identify additional cytokines from QFT supernatants for detection of Mycobacterium tuberculosis infection in children with tuberculosis and HIV co-infection from Ghana. Concentrations of 18 cytokines in QFT supernatants from children (0-16 years) with tuberculosis concomitantly infected with HIV (n = 25) or without HIV (n = 24) from Ghana were measured using cytometric bead array (CBA). 29% of the children showed positive IFN-γ test results, and five cytokines, i.e., IL-6, IL-21, TNF-α, IL-1α and IP-10, detected M. tuberculosis infection with comparable or, for IL-6, with significantly higher sensitivity (59%). Increased age and HIV co-infection were associated with decreased cytokine induction, and especially IL-21 and IP-10 were less prevalent in HIV co-infected children with tuberculosis. Combined cytokine analyses increased proportions of positive tests, and a four-cytokine subset (i.e., IL-6, IL-21, IFN-γ, IL-1α) predicted 78% of the children with tuberculosis correctly. Combined evaluation of IFN-γ and alternative cytokines improved IGRA-sensitivity in children with tuberculosis.</div></front></TEI><affiliations><list><country><li>Allemagne</li><li>Ghana</li></country><region><li>District de Düsseldorf</li><li>Rhénanie-du-Nord-Westphalie</li></region><settlement><li>Düsseldorf</li></settlement></list><tree><country name="Allemagne"><region name="Rhénanie-du-Nord-Westphalie"><name sortKey="Lundtoft, Christian" sort="Lundtoft, Christian" uniqKey="Lundtoft C" first="Christian" last="Lundtoft">Christian Lundtoft</name></region><name sortKey="Jacobsen, Marc" sort="Jacobsen, Marc" uniqKey="Jacobsen M" first="Marc" last="Jacobsen">Marc Jacobsen</name><name sortKey="Mayatepek, Ertan" sort="Mayatepek, Ertan" uniqKey="Mayatepek E" first="Ertan" last="Mayatepek">Ertan Mayatepek</name><name sortKey="Nausch, Norman" sort="Nausch, Norman" uniqKey="Nausch N" first="Norman" last="Nausch">Norman Nausch</name></country><country name="Ghana"><noRegion><name sortKey="Awuah, Anthony Afum Adjei" sort="Awuah, Anthony Afum Adjei" uniqKey="Awuah A" first="Anthony Afum-Adjei" last="Awuah">Anthony Afum-Adjei Awuah</name></noRegion><name sortKey="Enimil, Anthony" sort="Enimil, Anthony" uniqKey="Enimil A" first="Anthony" last="Enimil">Anthony Enimil</name><name sortKey="Owusu Dabo, Ellis" sort="Owusu Dabo, Ellis" uniqKey="Owusu Dabo E" first="Ellis" last="Owusu-Dabo">Ellis Owusu-Dabo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/SidaGhanaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000103 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000103 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= SidaGhanaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:28299430
|texte= Alternative Quantiferon cytokines for diagnosis of children with active tuberculosis and HIV co-infection in Ghana.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:28299430" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SidaGhanaV1
| This area was generated with Dilib version V0.6.31. |  |